Thermo Fisher Scientific H3K4me3 Polyclonal Antibody
H3K4me3에 특이적인 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC, IP, ChIP 등 다양한 응용 가능. 고순도 Affinity chromatography 정제, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Thermo Fisher Scientific H3K4me3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HIST3H3 (Accession Q16695), corresponding to amino acid residues M1–K24 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899178 |
Product Specific Information
Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys4.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can directly alter chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 can be modified by methylation, acetylation, or phosphorylation, influencing various cellular processes.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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