Thermo Fisher Scientific H3K27me3 Polyclonal Antibody
H3K27me3에 특이적으로 결합하는 Rabbit Polyclonal Antibody로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능하며, PBS/50% glycerol 용액 형태로 -20°C 보관됩니다. Affinity chromatography로 정제된 고품질 항체입니다.
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Thermo Fisher Scientific H3K27me3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HIST3H3 (Accession Q16695), corresponding to amino acid residues T12–G34 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899176 |
Product Specific Information
Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys27.
Target Information
H3K27me3 is an epigenetic mark where histone H3 is trimethylated at lysine residue 27. This modification is associated with transcriptional repression and is commonly found in heterochromatin regions and inactive gene promoters.
EZH2, a component of the PRC2 complex, acts as the methyltransferase responsible for H3K27 trimethylation, while UTX and JMJD3 serve as demethylases.
Regulation of H3K27me3 is essential for proper gene expression control, and its dysregulation is implicated in various diseases, including cancer.
H3K27me3 antibodies are widely used for western blot and ChIP assays to study histone modification patterns and gene regulation mechanisms.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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