Thermo Fisher Scientific H3K9me3 Polyclonal Antibody
Tri-Methyl-Histone H3 (Lys9) 특이적 인식 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용 가능. Rabbit Polyclonal 형태이며 Human, Mouse, Rat 등 반응성 보유. 고순도 Affinity chromatography 정제, PBS/glycerol buffer에 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human HIST3H3 (Accession Q16695), corresponding to amino acid residues M1–R27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899179 |
Product Specific Information
Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys9.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure by binding DNA. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 can undergo various post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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