Thermo Fisher Scientific PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Monoclonal Antibody (PCNA/6580)
PCNA 단백질을 인식하는 마우스 단클론 항체로 G1 및 S기 세포 증식 마커로 사용됩니다. Western blot, IHC 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 고순도 항체입니다. PBS 용액 형태로 제공되며 보존제를 포함하지 않습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCNA/6580 |
| Immunogen | Recombinant full-length human PCNA protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and plays a key role in DNA excision and mismatch repair pathways. It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells, particularly in G1 and S phases, and is useful for assessing tumor proliferation status.
Located in the nucleus, PCNA functions as a cofactor of DNA polymerase delta, forming a homotrimer that enhances leading strand synthesis during replication.
Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein are known, and pseudogenes exist on chromosomes 4 and X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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