Thermo Fisher Scientific Human LIF Recombinant Protein
인간 유래 LIF 재조합 단백질로, E. coli 발현 시스템에서 생산된 고순도(≥95%) 단백질입니다. 세포 증식 기능 분석 및 줄기세포 연구용으로 적합하며, ED50 ≤ 0.05 ng/mL의 높은 생물학적 활성을 보입니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- PHC948x (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human LIF Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF |
| Molecular Weight | 21 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 0.05 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Dry ice |
Product Specific Information
Reconstitution:
Centrifuge the vial briefly before opening to bring contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Apportion the reconstituted protein into working aliquots and store at -20°C. Make further dilutions in buffered solution containing a carrier protein, such as PBS with 0.1% BSA.
Storage:
Store the lyophilized protein at 2–8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Leukemia inhibitory factor (LIF) is a 20 kDa protein belonging to the IL-6 receptor family. It binds to a heterodimeric membrane receptor composed of a LIF-specific subunit (gp190 or LIFR) and the shared subunit gp130. LIF expression occurs in various tissues, including thymus, lung, and neuronal tissue. It can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels are found in rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF is known for inhibiting embryonic stem cell differentiation in mice and promoting stem cell self-renewal. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity, though stem cell differentiation inhibition is mouse-specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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