Thermo Fisher Scientific Human M-CSF Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control (Ctrl): Assay-dependent

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Product Specifications

Product Specific Information

Reconstitution:
Lyophilized human M-CSF should be reconstituted in 20 mM Tris-HCl, pH 8.0 to a concentration of 0.1–1.0 mg/mL.
For extended storage, further dilute in a buffer containing a carrier protein (e.g., 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage:
Store lyophilized human M-CSF at 2°C to 8°C, preferably desiccated.
Reconstituted protein should be stored at ≤ -20°C (not in a frost-free freezer).
Minimize freeze-thaw cycles.

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is an essential survival factor for proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. It also induces VEGF secretion by macrophages, promoting endothelial progenitor cell mobilization and neovascularization.
M-CSF exists in several bioactive isoforms differing in potency and stability. The full-length protein is membrane-spanning and may be cleaved and modified in secretory vesicles. It forms a disulfide-bonded homodimer, processed into glycoprotein or proteoglycan isoforms via chondroitin sulfate modification.
Binding to its receptor c-Fms (CSF-1R/CD115) induces receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations.
Human M-CSF is active on mouse cells, whereas mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.