Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), Alexa Fluor 488, eBioscience
Alexa Fluor 488로 결합된 Alpha-Smooth Muscle Actin 단클론 항체(1A4)는 인간, 마우스, 랫트 시료에서 α-SMA를 검출합니다. IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 4°C 암소 보관이 권장됩니다. 세포 골격 연구 및 종양 표지 분석에 유용합니다.
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Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), Alexa Fluor 488, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 23 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–5 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 16 publications |
| Flow Cytometry (Flow) | - | View 12 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A4 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574460 |
Product Specific Information
The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin (α-SMA), a 42 kDa protein and major component of the cytoskeletal structural network. This antibody is specific for the alpha form of muscle actin expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. It is also expressed in various tissue parenchyma and stroma.
Alpha-smooth muscle actin is useful for identifying leiomyomas, leiomyosarcomas, pleomorphic adenomas, and serves as a prognostic marker for basal cell carcinoma.
Applications Reported:
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
- Immunocytochemistry
Applications Tested:
- IHC (P): ≤1 µg/mL using low pH antigen retrieval
- ICC: ≤1 µg/mL using methanol-fixed cells
Careful titration is recommended for optimal performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Smooth Muscle Actin belongs to the actin family, which plays a critical role in cell motility, structure, and integrity. Alpha, beta, and gamma actin isoforms are identified, with alpha actin being a major constituent of the contractile apparatus. Smooth muscle actin (α-actin) is found in skeletal muscle and smooth muscle vessel walls, gut wall, myometrium, and myoepithelial cells.
Defects in the smooth muscle actin gene can cause familial thoracic aortic aneurysm type 6. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells and appears in fibroblastic stress fibers during wound healing and fibrocontractive diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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