Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (5H10), APC-Alexa Fluor 750
APC-Alexa Fluor 750 형광으로 표지된 CD8α 단클론 항체(클론 5H10). Flow cytometry에 최적화되어 있으며 Mouse 시료에 반응. Rat IgG2b 아이소타입, PBS/BSA 버퍼에 보존, 4°C 보관. 면역세포 표지 및 T세포 분석에 적합.
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Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (5H10), APC-Alexa Fluor 750
Applications
- Flow Cytometry (Flow): Assay-dependent
View 5 publications - Miscellaneous PubMed (Misc): -
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5H10 |
| Immunogen | Con A-stimulated BALB/c splenic T cells |
| Conjugate | APC-Alexa Fluor™ 750 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10374588 |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor 700
- APC
- FITC
- Pacific Blue
- Pacific Orange
- PE
- PE-Cyanine7
- PE-Texas Red
- PerCP
- Request custom conjugation
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It plays a key role in signal transduction, T cell differentiation, and activation.
CD8 is mainly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with TCR for MHC class I molecules.
The CD8α chain is essential for MHC-I binding and is also found on subsets of T cells, NK cells, monocytes, and dendritic cells as homodimers.
Engagement of MHC-I/peptide complexes triggers Lck kinase recruitment, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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