Thermo Fisher Scientific VIM Monoclonal Antibody (OTI3B11), TrueMAB
Human Vimentin 단백질(AA 210-466)에 특이적인 Mouse Monoclonal Antibody. Western blot 검증 완료. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 구성. 연구용으로 세포 골격 단백질 분석에 적합.
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Applications
- Western Blot (WB): 1:200–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210–466 of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in mesenchymal cells. It plays a role in intracellular protein transport between the nucleus and plasma membrane.
During early cellular development, vimentin is co-expressed with desmin and later replaced by tissue-specific intermediate filament proteins.
Vimentin is implicated in steroid synthesis regulation through its function as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase triggers intermediate filament breakdown, facilitating lipid droplet–mitochondria interaction and enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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