Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), NovaFluor Red 685, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: ≤ 0.6 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• Brilliant UV 661 / 737
• Brilliant Violet 421 / 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S / Red 710 / Red 725 / Red 755 / Yellow 610
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Custom Conjugation Available

Product Specific Information

The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells, leukocytes, and platelets. CD31 mediates cell-cell and cell-matrix interactions, adhesion, and signal transduction.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Flow cytometric analysis
• Immunohistochemical staining of frozen tissue sections

Applications Tested

• Flow cytometry: ≤ 0.6 µg/test (100 µL sample volume)
• Immunohistochemistry (frozen tissue): ≤ 20 µg/mL
• Optimal performance requires careful titration.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.

CellBlox Plus Blocking Buffer

Updated conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
Use 5 µL per sample (10³–10⁸ cells) for optimal staining.
Add to antibody cocktails before combining with cells.

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for improved spectral performance and reduced spillover.
Excitation: 637 nm; Emission: 685 nm; Laser: 633–640 nm (Red).

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs. It is expressed throughout the vascular compartment, primarily at intercellular junctions.
Functions include:

• Homophilic binding for cell-cell interaction
• Regulation of integrin-mediated adhesion and transendothelial migration
• Roles in angiogenesis, apoptosis, immune regulation, and thrombosis

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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