Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The GL1 monoclonal antibody reacts with mouse CD86 (B7-2), an ~80 kDa surface receptor and member of the B7 costimulatory family. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated through stimuli such as BCR complex, CD40, and cytokine receptors. It is also expressed by activated mouse T cells and peritoneal cells.

CD86 interacts with T-cell surface molecules CD28 and CTLA-4 (CD152), playing a critical role in T-B cell crosstalk, T-cell costimulation, autoantibody production, and Th2-mediated Ig production. Upregulation kinetics indicate CD86’s major contribution during the primary immune response phase.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry on stimulated mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume). Optimal titration required.

Fluorochrome Information:
Super Bright 436 is excited by a 405 nm violet laser and emits at 436 nm. Recommended detection with a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD86, along with CD80, serves as a ligand for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Insufficient co-stimulation via CD86/CD80 can induce tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells.

Binding of CD86 with CD28 provides a costimulatory signal for T-cell activation, whereas binding with CTLA-4 inhibits T-cell activation, reducing immune response. Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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