Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Super Bright 436, eBioscience
마우스 CD90.2(Thy-1.2)를 인식하는 53-2.1 단클론 항체로, Super Bright 436 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기됩니다. BALB/c, C57BL/6 등 CD90.2 발현 마우스 균주 분석에 적합합니다.
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Applications
Immunohistochemistry (IHC)
- Tested Dilution: Not specified
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 0.03 µg/test
- View 7 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688052 |
Additional Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 555
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 760
- NovaFluor Red 710
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains such as BALB/c, CBA, C3H, C57BL/6, C58/, and SJL. Cells from CD90.1-expressing strains (e.g., PL, AKR) do not stain with 53-2.1.
CD90 is involved in T-cell adhesion and signal transduction regulation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.03 µg/test (100 µL final volume). Optimize titration for best performance.
Fluorochrome properties:
- Excitation: 405 nm
- Emission: 436 nm
- Recommended filter: 450/50 bandpass
- Laser: Violet
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD90.2 (Thy-1) is a GPI-linked glycoprotein member of the immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
CD90.2 plays roles in cell-cell and cell-ligand interactions during synaptogenesis and other neural processes.
It is an 18 kDa plasma membrane antigen expressed by hematopoietic and neuronal cells, connective tissue, fibroblast and stromal lines, and tumor endothelial cells.
In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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