Thermo Fisher Scientific HIF-2 alpha Monoclonal Antibody (ep190b)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:150-1:300

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

2-5 µg/mL

-

Flow Cytometry (Flow)

1:400

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

Assay-Dependent

-

ChIP assay (ChIP)

Assay-Dependent

-

Gel Shift (GS)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ep190b

Immunogen

Synthetic peptid corresponding to residues 535-631 of human HIF-2 alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF-2 alpha,uniProtId:Q99814-1,ncbiNodeId:9606,antigenRange:535-631,antigenLength:870,antigenImageFileName:MA1-16519_HIF-2_alpha_Q99814-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16519_HIF-2_alpha_Q99814-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• DyLight 550
• DyLight 650
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2098238

Product Specific Information

Suggested positive control: antigen standard for EPAS1 (transient overexpression lysate).

Target Information

HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.