Thermo Fisher Scientific HIF-2 alpha Monoclonal Antibody (ep190b)
HIF-2 alpha 단백질을 인식하는 Mouse monoclonal antibody(ep190b). Western blot, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합. Human 등 여러 종에 반응하며, Protein G로 정제된 액상형 항체. VEGF 발현 및 산소 반응 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | 1 publication available |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150–1:300 | |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 2–5 µg/mL | |
| Flow Cytometry (Flow) | 1:400 | |
| ELISA | 1:100–1:2,000 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| ChIP assay (ChIP) | Assay-dependent | |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ep190b |
| Immunogen | Synthetic peptide corresponding to residues 535–631 of human HIF-2 alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2098238 |
Available Conjugate Formats
Product Specific Information
Suggested positive control: antigen standard for EPAS1 (transient overexpression lysate).
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of oxygen-regulated genes. It contains a basic-helix-loop-helix domain and dimerization domain, participating in oxygen-sensing signal transduction pathways. HIF-2 alpha regulates VEGF expression and is implicated in angiogenesis and lung tubular system development. Mutations in this gene are associated with familial erythrocytosis type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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