Thermo Fisher Scientific HIF1A Monoclonal Antibody (H1alpha67)
HIF1A 단백질을 인식하는 Mouse Monoclonal Antibody (Clone H1alpha67). Western blot, IHC, ICC, Flow 등 다양한 응용에 사용 가능. 인간 및 여러 종에 반응. 단백질 G 정제, PBS + 1% BSA 저장, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | 16 publications |
| Immunohistochemistry (IHC) | - | 39 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 | 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20–1:50 | - |
| Immunohistochemistry - Free Floating (IHC (Free)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:50 | 1 publication |
| Flow Cytometry (Flow) | 1:10–1:1,000 | - |
| ELISA | 1:100–1:2,000 | - |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
| ChIP assay (ChIP) | 1–5 µg | 1 publication |
| in situ PLA (PLA) | Assay-Dependent | - |
| Gel Shift (GS) | 1:1–1:100 | 3 publications |
| In vitro Assay (IV) | Assay-Dependent | - |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus |
| Published Species | Bovine, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1alpha67 |
| Immunogen | Fusion protein containing residues 432–528 of human HIF-1α |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568567 |
Available Conjugated Formats
Product Specific Information
- In Western blot, multiple bands may appear at 100–120 kDa, representing post-translational modifications of HIF-1α.
- For WB, testing on nuclear extracts is recommended.
- Recommended positive controls:
COS-7 nuclear hypoxic induced cell lysate, HeLa hypoxic (CoCl₂) cell lysate, HepG2 hypoxic (CoCl₂) cell lysate, HeLa hypoxic cell lysate, HepG2 hypoxic cell lysate, HIF-1α Knockout HeLa cell lysate.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors.
HIF1 acts as a transcriptional regulator of the adaptive response to hypoxia, activating over 40 genes such as erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and p300/CBP complexes to modulate hypoxia-responsive gene expression.
Located on chromosome 14q in humans, HIF1A plays a critical role in diseases such as ischemia, cancer, pulmonary hypertension, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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