Thermo Fisher Scientific HIF1A Monoclonal Antibody (H1alpha67)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 39 publications 39 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:20-1:50

-

Immunohistochemistry - Free Floating (IHC (Free))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:1,000

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

1:10-1:500

-

ChIP assay (ChIP)

1-5 µg

View 1 publication 1 publication

in situ PLA (PLA)

Assay-Dependent

-

Gel Shift (GS)

1:1-1:100

View 3 publications 3 publications

In vitro Assay (IV)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Cat, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus

Published species

Bovine, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

H1alpha67

Immunogen

Fusion protein containing residues 432-528 of human HIF-1alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1A,uniProtId:Q16665-1,ncbiNodeId:9606,antigenRange:432-528,antigenLength:826,antigenImageFileName:MA1-16504_HIF1A_Q16665-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16504_HIF1A_Q16665-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• DyLight 488
• DyLight 550
• DyLight 650
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568567

Product Specific Information

In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.

For WB, testing on nuclear extracts is recommended.

Recommended positive controls: COS-7 nuclear hypoxic induced cell lysate, HeLa hypoxic (CoCl2) cell lysate, HepG2 hypoxic (CoCl2) cell lysate, HeLa hypoxic cell lysate, HepG2 hypoxic cell lysate, HIF-1 alpha Knockout HeLa cell lysate

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.