Thermo Fisher Scientific HIF1A Monoclonal Antibody (ESEE122)
HIF1A 단백질을 검출하기 위한 Thermo Fisher Scientific의 단클론 항체(ESEE122). 다양한 종에 반응하며 WB, IHC, ICC, Flow 등 여러 응용에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, PBS 버퍼에 0.02% sodium azide 포함. 연구용으로만 사용.
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Thermo Fisher Scientific HIF1A Monoclonal Antibody (ESEE122)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:5,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | 1:10–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ESEE122 |
| Immunogen | Residues 329–530 of human HIF-1 alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_568569 |
Additional Formats
Product Specific Information
Suggested positive control: Cos7 CoCl₂-treated nuclear extract.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under low oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the PAS family of transcription factors. It regulates the adaptive response to hypoxia by activating transcription of genes involved in oxygen delivery and metabolic adaptation, such as erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha also plays a role in hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Under hypoxic conditions, HIF1-alpha interacts with p53 and undergoes MDM2-mediated ubiquitination and proteasomal degradation. Induction of NOX4 by HIF1-alpha helps maintain ROS levels after hypoxia. In humans, HIF1A is located on chromosome 14q, and its C-terminal region binds to p300, forming complexes that induce hypoxia-responsive genes.
Hypoxia contributes significantly to diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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