Thermo Fisher Scientific NEDD8 Monoclonal Antibody (1A7)
인간 NEDD8 단백질을 특이적으로 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용 가능. 고순도 Protein G 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A7 |
| Immunogen | Purified recombinant fragment of human NEDD8 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538604 |
Product Specific Information
MA5-17133 targets NEDD8 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human NEDD8 expressed in E. coli.
MA5-17133 detects NEDD8 with a predicted molecular weight of approximately 9 kDa.
Target Information
NEDD8 is a ubiquitin-like protein that plays an important role in cell cycle control and embryogenesis.
Covalent attachment to its substrates requires prior activation by the E1 complex (UBE1C-APPBP1) and linkage to the E2 enzyme (UBE2M).
Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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