Thermo Fisher Scientific NEFL Monoclonal Antibody (1H3)
인간 NEFL 단백질을 검출하는 마우스 모노클로날 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용 가능. 비결합형 액상 형태로 제공되며, 장기 보관 시 -20°C 권장. 신경세포 내 신경필라멘트 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H3 |
| Immunogen | Purified recombinant fragment of human NEFL expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2538606 |
Product Specific Information
MA5-17135 targets NEFL in indirect ELISA, FACS, ICC, IHC, IF, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human NEFL expressed in E. coli.
MA5-17135 detects NEFL with a predicted molecular weight of approximately 62 kDa.
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons and are crucial for maintaining neuronal caliber. They consist mainly of three major proteins: NF-L, NF-M, and NF-H.
NF-L is the light or low molecular weight subunit (~70 kDa), while NF-H is the heavy subunit (~180–220 kDa).
Expression of these proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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