Thermo Fisher Scientific Nestin Monoclonal Antibody (1A9G12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

1A9G12

Immunogen

Purified recombinant fragment of human NES (amino acids: 419-588 ) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nestin,uniProtId:P48681-1,ncbiNodeId:9606,antigenRange:419-588,antigenLength:1621,antigenImageFileName:MA5-17136_Nestin_P48681-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17136_Nestin_P48681-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538607

Product Specific Information

MA5-17136 targets NES in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17136 immunogen is purified recombinant fragment of human NES (amino acids: 419-588 ) expressed in E. Coli.

MA5-17136 detects NES which has a predicted molecular weight of approximately 177kDa.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.