Thermo Fisher Scientific NCK1 Monoclonal Antibody (5B7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B7

Immunogen

Purified recombinant fragment of human NCK1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538603

Product Specific Information

MA5-17132 targets NCK1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-17132 immunogen is purified recombinant fragment of human NCK1 expressed in E. Coli.

MA5-17132 detects NCK1 which has a predicted molecular weight of approximately 47kDa.

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.