Thermo Fisher Scientific CD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells, and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and serves as a potent costimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.

Applications Reported:

• Flow cytometric analysis
• T cell costimulation in vitro and in vivo

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes
• Recommended ≤0.5 µg per test (100 µL final volume)
• Optimal antibody titration should be determined empirically

Storage and Handling:

• Use in a sterile environment
• 0.2 µm post-manufacturing filtered

Quality Control:

• Purity: >90% (SDS-PAGE)
• Endotoxin Level: <0.001 ng/µg (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein and a member of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and functions as a key costimulatory receptor by binding to CD80 and CD86 on antigen-presenting cells.

CD28 prevents T cells from entering an anergic or apoptotic state and plays a critical role in T cell activation and survival. In murine tissues, CD28 is expressed on all CD4+ and CD8+ T cells, with varying expression levels during thymocyte development.

Diseases associated with CD28 dysfunction include mycosis fungoides and Sezary’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.