Thermo Fisher Scientific CD28 Monoclonal Antibody (37.51), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 64 publications 64 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 79 publications 79 publications

Inhibition Assays (IA)

-

View 1 publication 1 publication

T-Cell Activation (TCA)

-

View 8 publications 8 publications

Affinity Purification (AP)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

37.51

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Unconjugated
• APC
• Biotin
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865678

Product Specific Information

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis. 37.51 has also been reported in costimulation of T cells in vitro and in vivo .

Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary`s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.