Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI1D2), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:200–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) before use.

Target Information

MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways and essential for glucose transporter expression. It phosphorylates and activates MAPK14/p38-MAPK and can be activated by insulin. Expression of the RAS oncogene leads to accumulation of active MAP2K3, resulting in constitutive activation of MAPK14 and oncogenic transformation of primary cells. Inhibition of this kinase is implicated in the pathogenesis of Yersinia pseudotuberculosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported for MEK3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.