Thermo Fisher Scientific DDX5 Monoclonal Antibody (Ab204), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/ml

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Ab204

Immunogen

Purified T antigen-related protein from Ad2+ ND2 virus-infected HeLa cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DDX5,uniProtId:P17844-1,ncbiNodeId:9606,antigenRange:1-614,antigenLength:614,antigenImageFileName:14-6005-82_DDX5_P17844-1_House_mouse.svg,antigenImageFileNamePDP:14-6005-82_DDX5_P17844-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 647
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802195

Product Specific Information

Description: This Ab204 monoclonal antibody detects human DDX5 protein also known as DEAD box protein 5 and RNA helicase p68. This antibody has been reported to cross-react with mouse and rat orthologs of DDX5 protein.

Applications Reported: This Ab204 antibody has been reported for use in western blot and Immunocytochemistry. (Fluorochrome-conjugated Ab204 is recommended for use in flow cytometry.)

Applications Tested: This Ab204 antibody has been tested by western blot of mouse lymph node cells. This may be used at less than or equal to 4.0 µg/mL.

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.