Thermo Fisher Scientific MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), eBioscience

Thermo Fisher Scientific MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test
• Reported for: Flow cytometric analysis, immunohistochemical staining (frozen mouse tissue sections), complement-dependent cytotoxicity
• Tested on: Mouse splenocytes
• Recommended to titrate for optimal performance

Product Specifications

Available Conjugated Formats

• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585 / 610-70S / 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 570 / 660
• PE
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

• Description: The 34-1-2S monoclonal antibody reacts with mouse MHC class I molecules H-2Kd and H-2Dd. It also cross-reacts with Kb, s, r, q, and p.
• Purity: Greater than 90%, determined by SDS-PAGE
• Aggregation: Less than 10%, determined by HPLC
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

This protein is a mouse MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are essential in Immunopeptidomics, enabling identification and characterization of neoantigens via HPLC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.