Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience

Applications and Tested Dilution

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Product Specifications

Available Conjugated Formats

• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available upon request

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Applications Reported:
Flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation, and complement-dependent cytotoxicity.

Applications Tested:
Validated in flow cytometric analysis of mouse splenocytes. Recommended usage ≤1 µg per test (100 µL final volume). Cell number may range from 10^5 to 10^8 per test. Antibody should be titrated for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are essential tools in Immunopeptidomics, supporting the identification and characterization of neoantigens via HPLC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.