Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience
마우스 MHC Class I(H-2Db)을 인식하는 단클론 항체로, Flow Cytometry, IHC, Immunoprecipitation 등에 사용됩니다. 다양한 형광 및 비오틴 결합 형식으로 제공되며, 고순도(>90%)와 낮은 응집도(<10%)를 보장합니다. 연구용으로만 사용 가능합니다.
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- 14-5999-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 28-14-8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467825 |
Available Conjugated Formats
- APC
- Biotin
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510
- NovaFluor Blue 585
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 710
- NovaFluor Red 755
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Custom conjugation available upon request
Product Specific Information
The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.
Applications Reported:
Flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, immunoprecipitation, and complement-dependent cytotoxicity.
Applications Tested:
Validated in flow cytometric analysis of mouse splenocytes. Recommended usage ≤1 µg per test (100 µL final volume). Cell number may range from 10^5 to 10^8 per test. Antibody should be titrated for optimal performance.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is an MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are essential tools in Immunopeptidomics, supporting the identification and characterization of neoantigens via HPLC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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