Thermo Fisher Scientific RIP3 Polyclonal Antibody, eBioscience
Rabbit polyclonal antibody specific for mouse and rat RIP3 protein. Validated for Western blot and IHC applications. High purity (>90%) and low aggregation (<10%). Supplied as liquid, 0.5 mg/mL concentration, stored at 4°C. For research use only.
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- 14-6048-82
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–4 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10853961 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
This rabbit polyclonal antibody reacts with mouse and rat Receptor Interacting Protein (RIP) 3, a 57 kDa Ser/Thr kinase expressed ubiquitously in the cytoplasm. RIP3 can shuttle between nucleus and cytoplasm and binds to and phosphorylates RIP1, inhibiting RIP1- and TNFR1-mediated NFκB activation. It mediates programmed necrosis through regulation of TNF-induced reactive oxygen species production and acts as a molecular switch between TNF-induced apoptosis and necrosis.
Applications Reported: Western blotting and immunohistochemistry.
Applications Tested: Validated by western blot analysis using NIH-3T3 cell lysate (0.5–4 µg/mL recommended). Optimize titration for best assay performance.
Target Information
RIPK3 is a serine/threonine kinase of the receptor-interacting protein family, possessing a unique C-terminal domain. It is mainly localized in the cytoplasm and mitochondria, capable of nucleocytoplasmic shuttling via specific localization signals. RIPK3 is part of the TNF receptor-I signaling complex, inducing apoptosis and weak NF-κB activation. Caspase-8 prevents RIPK3-dependent necrosis by forming a proteolytically active complex with CFLAR, essential for protective function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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