Thermo Fisher Scientific SUMO1 Monoclonal Antibody (1SUM-1C6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

ELISA (ELISA)

1:500-1:5,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1SUM-1C6

Immunogen

Ovalbumin conjugated synthetic peptide corresponding to aa 85 to aa 97 of human SUMO1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SUMO1,uniProtId:P63165-1,ncbiNodeId:9606,antigenRange:85-97,antigenLength:101,antigenImageFileName:MA3-088_SUMO1_P63165-1_House_mouse.svg,antigenImageFileNamePDP:MA3-088_SUMO1_P63165-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2633333

Product Specific Information

MA3-088 detects SUMO1 from human and mouse samples. The immunogen sequence is conserved in SUMO1 of mouse origin.

MA3-088 has been successfully used in Western blot, immunofluorescence and ELISA applications.

Ovalbumin conjugated synthetic peptide (C)-EDVIEVYQEQTGG) corresponding to aa 85-97 of human SUMO1.

Target Information

SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.