Thermo Fisher Scientific CD31 Monoclonal Antibody (HEC7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 10 publications 10 publications

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

View 5 publications 5 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Bovine, Human

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

HEC7

Immunogen

Human PECAM-1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223516

Product Specific Information

MA3100 targets CD31 in ICC/IF and ACS applications and shows reactivity with Human and Bovine samples.

The MA3100 immunogen is human PECAM-1.

MA3100 detects CD31 which has a predicted molecular weight of approximately 80 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.