Thermo Fisher Scientific Phospho-Caveolin 2 (Tyr19) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues M(14) A D D A (pY) S H H S G C(25) of mouse CAV2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caveolin 2,uniProtId:Q9WVC3-1,ncbiNodeId:10090,antigenRange:14-25,antigenLength:162,antigenImageFileName:PA1-060_Caveolin_2_Q9WVC3-1_Rabbit.svg,antigenImageFileNamePDP:PA1-060_Caveolin_2_Q9WVC3-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2072311

Product Specific Information

PA1-060 detects phospho-caveolin-2 Y19 from human and mouse samples.

PA1-060 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~21 kDa protein representing phospho-caveolin-2 Y19 from extract from COS-7 cells transiently transfected with the mouse caveolin-2 gene. Immunofluorescent staining of phospho-caveolin-2 Y19 in NIH 3T3 cells using PA1-060 results in a distinct punctate staining pattern at the cell periphery.

The PA1-060 immunogen is a synthetic phosphopeptide corresponding to residues M(14) A D D A (pY) S H H S G C(25) of mouse CAV2. This peptide (Cat. # PEP-180) is available for use in neutralization and control experiments.

Target Information

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.