Thermo Fisher Scientific Adiponectin Receptor 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Adiponectin Receptor 1,uniProtId:Q96A54-1,ncbiNodeId:9606,antigenRange:21-37,antigenLength:375,antigenImageFileName:PA1-059_Adiponectin_Receptor_1_Q96A54-1_Rabbit.svg,antigenImageFileNamePDP:PA1-059_Adiponectin_Receptor_1_Q96A54-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2221905

Product Specific Information

PA1-059 detects human and rat adiponectin receptor 1.

PA1-059 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~42 kDa protein corresponding to human adiponectin receptor 1 from transfected hepatocytes.

The PA1-059 immunogen is a synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1.

Target Information

Adiponectin (also known as 30- kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR-. Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.