Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test
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Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 647
• APC
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily containing an ITIM domain.
KLRG1 is expressed on subsets of γδ T cells, NK (CD56dim), CD8⁺, and CD4⁺ T cells, primarily by effector/memory phenotype cells that are short-lived but capable of immediate effector function.
Cadherin-E, -N, and -R are ligands for KLRG1; their interaction inhibits cytolytic activity and proliferation.
Clone 13F12F2 and 13A2 recognize similar epitopes based on cross-blocking studies.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry of normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.

Fluorochrome Information:
APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Ensure the instrument can detect this fluorochrome.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation; protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation / Emission: 633–647 nm / 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in the cytoplasmic domain.
These ITIMs interact with SH2 domains of protein phosphatases such as SHP-1.
KLRG1 is expressed on NK cells and certain T cells, which are involved in lysis of tumor and virus-infected cells, mediating humoral and cell-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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