Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), DyLight 650
인간 Nestin 단백질에 특이적인 마우스 모노클로날 항체로 DyLight 650 형광 표지. 다양한 응용 분야(WB, IHC, ICC, Flow)에 사용 가능. 인간 시료에 반응하며 설치류 및 토끼 시료와 교차 반응 없음. 단백질 G 정제, 4°C 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | Fragment of human Nestin protein (residues 1464–1614) |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 1.58 mg/mL |
| Purification | Protein G |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152637 |
Available Formats
Product Specific Information
This antibody does not cross-react with rodent or rabbit.
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It serves as a key marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also recognized as a superior angiogenic marker for assessing neovascularity in tumor endothelial cells.
Highly specific antibodies to human Nestin can assist in characterizing progenitor cells differentiating into distinct lineages, enhancing therapeutic potential of human neural stem/progenitor cells for CNS disease or injury, and identifying neuroepithelial tumor cells.
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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