Thermo Fisher Scientific Glucose 6-Phosphate Isomerase Monoclonal Antibody (CPTC-GPI-1)
Glucose 6-Phosphate Isomerase 단백질을 인식하는 Mouse monoclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. Recombinant human GPI를 면역원으로 제작. 단백질 A/G 정제, PBS 용액 형태로 제공. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-GPI-1 |
| Immunogen | Recombinant human full-length GPI protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The GPI gene encodes a multifunctional phosphoglucose isomerase involved in cellular energy pathways.
This dimeric enzyme catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate.
In the cytoplasm, it participates in glycolysis and gluconeogenesis, while extracellularly it acts as a neurotrophic factor for spinal and sensory neurons.
Mutations in this gene are associated with nonspherocytic hemolytic anemia, hydrops fetalis, neonatal death, and neurological impairment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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