Thermo Fisher Scientific ERp57 Monoclonal Antibody (4F9), PerCP
ERp57 단백질을 인식하는 Mouse Monoclonal Antibody (Clone 4F9), PerCP 결합 형식. Western blot 및 Immunocytochemistry에 적합. 482/675 nm 형광 특성. Protein G 정제, 4°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F9 |
| Immunogen | Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25–505 (His-tagged) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931830 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45376 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Detects approximately 57 kDa protein.
Target Information
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins.
The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity.
It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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