Thermo Fisher Scientific CD74 Monoclonal Antibody (3D7), APC

Applications and Tested Dilution

Product Specifications

Available Formats

• FITC (Cat. No. MA5-45382)
• PerCP (Cat. No. MA5-45383)
• Custom conjugation available upon request

Product Specific Information

A 1:1,000 dilution of MA5-45381 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 33 kDa protein.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene contains 5 exons:

• Exon 1 encodes the leader peptide
• Exons 2 and 3 encode the two extracellular domains
• Exon 4 encodes the transmembrane domain and cytoplasmic tail

DRA does not have polymorphisms in the peptide-binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
Within the DR molecule, the beta chain contains all the polymorphisms specifying peptide-binding specificities.
Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.

HLA-DR is expressed primarily on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
Three loci — DR, DQ, and DP — encode the major expressed products of the human class II region.
The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and play a critical role in initiating the immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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