Thermo Fisher Scientific ERp57 Monoclonal Antibody (4F9), FITC
ERp57 단백질을 인식하는 FITC 결합 단클론 항체로, Western Blot 및 면역형광(ICC/IF)에 적합합니다. 인간 시료에 반응하며, Protein G로 정제된 액상 형태입니다. 4°C 암소 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F9 |
| Immunogen | Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25–505 (his-tagged) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.4) with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931829 |
Additional Formats
- Unconjugated (MA5-27689)
- APC (MA5-45374)
- PE (MA5-45377)
- PerCP (MA5-45376)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45375 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 57 kDa protein.
Target Information
The ERp57 gene encodes an endoplasmic reticulum protein that interacts with lectin chaperones calreticulin and calnexin to assist in the folding of newly synthesized glycoproteins. Although once thought to be a phospholipase, it has been shown to have protein disulfide isomerase activity. Complexes of lectins and ERp57 promote proper disulfide bond formation in glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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