Thermo Fisher Scientific Glucagon Monoclonal Antibody (2F9)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-15794 targets GCG in indirect ELISA and WB applications, showing reactivity with Human samples.
The immunogen is a purified recombinant fragment of human GCG expressed in E. coli.
MA5-15794 detects GCG, which has a predicted molecular weight of approximately 21 kDa.

Target Information

Glucagon is a 29-residue polypeptide hormone (MW 3482) produced in the pancreas.
A related hormone, enteroglucagon (or oxyntomodulin), produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus.

The biological activities of pancreatic glucagon include:

• Glycogenolysis
• Lipolysis
• Gluconeogenesis
• Ketogenesis

These actions are antagonistic to insulin, leading to increased blood glucose levels.
Immunocytochemical studies have shown glucagon presence in A (alpha) cells, which make up 15–20% of islet cells and are located at the periphery of pancreatic islets.

Pathological manifestations of glucagon-type peptides are mainly associated with tumors or glucagonomas, as no glucagon-cell deficiency or hyperplasia states have been identified.
Glucagon-specific antibodies are useful as cell and tumor markers in immunohistochemistry and as analytical tools for hormone quantification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.