Thermo Fisher Scientific CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), FITC, eBioscience

Applications

Immunohistochemistry (IHC)

• Not tested
  View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test
  View 13 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• PE
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein, a critical component of the T-cell receptor (TCR) complex and a marker of the T-cell lineage. CD3 participates in signal transduction from the TCR to the nucleus, leading to IL-2 transcription and clonal expansion.

In vitro, immobilized G4.18 antibody activates T cells, while soluble G4.18 inhibits allogeneic mixed-lymphocyte proliferation and cytotoxicity. In vivo, it induces long-term tolerance to organ allografts.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on rat splenocytes at ≤0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best results.
Excitation/Emission: 488 nm / 520 nm (Blue Laser).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cells and regulates TCR expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded on human chromosome 11. These subunits associate with intracellular signaling molecules via tyrosine-based motifs.

CD3 is expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. Abnormal T-cell percentages are observed in some autoimmune diseases. Mutations in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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