Thermo Fisher Scientific Rtf1 Polyclonal Antibody
Rtf1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot 및 Immunoprecipitation에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 인간 단백질에 반응합니다. 4°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:10,000–1:30,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 620 and 670 of Human Restores TBP (TATA box-binding protein) function-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65, and gammaPAK p62, exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste20, involved in pheromone signaling.
The alpha, beta, and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and leading to autophosphorylation.
There are eight autophosphorylation sites on gammaPAK, including Ser19, Ser141, and Thr402. Phosphorylation of Ser141 and Thr402 correlates with gammaPAK activation. Once phosphorylated, the PAK isoforms dissociate from Rac/Cdc42 to interact with downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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