Thermo Fisher Scientific Rtf1 Polyclonal Antibody
Rtf1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot 및 Immunoprecipitation에 사용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C 보관 및 Wet ice 배송. Mouse와 Orangutan에서도 반응 예측.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 300 and 350 of Human Restores TBP (TATA box-binding protein) function-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- 권장 유효기간: 수령일로부터 1년
- 100% 서열 동일성 기준으로 Mouse 및 Orangutan에서도 반응이 예측됨
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling.
The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation.
There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation.
Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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