Thermo Fisher Scientific Rtf1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:10,000–1:30,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Mouse

Target Information

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65, and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste20, involved in pheromone signaling.
The alpha, beta, and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and leading to their autophosphorylation.
There are eight sites of autophosphorylation on gammaPAK, including Ser19, Ser141, and Thr402, and phosphorylation of Ser141 and Thr402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.