Thermo Fisher Scientific Rtf1 Polyclonal Antibody
Rtf1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 Immunoprecipitation에 적합합니다. Human에 반응하며 Mouse, Orangutan에도 예측 반응합니다. 항원 친화 크로마토그래피로 정제되었으며 1 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 300–350 of Human Restores TBP (TATA box-binding protein) function-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65, and gammaPAK p62, exhibit high sequence homology with the S. cerevisiae kinase Ste20, involved in pheromone signaling.
The alpha, beta, and gammaPAK isoforms specifically complex with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and leading to autophosphorylation.
There are eight sites of autophosphorylation on gammaPAK, including Ser19, Ser141, and Thr402, with phosphorylation of Ser141 and Thr402 correlated with gammaPAK activation.
Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms dissociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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