Thermo Fisher Scientific Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody (SU0338)
Cytokeratin 8 단백질을 인식하는 recombinant rabbit monoclonal 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 높은 특이성과 재현성, lot 간 일관성 제공. 인체 및 마우스 시료에 반응.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,500 | - |
| Immunocytochemistry (ICC/IF) | 1:400–1:800 | View 3 publications |
| Flow Cytometry (Flow) | 1:500–1:1,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU0338 |
| Immunogen | Synthetic peptide within Human Cytokeratin 8 (aa 321–370) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809410 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Cytokeratin 8 is a member of the type II keratin family located on chromosome 12. It heteropolymerizes with type I keratins to form intermediate filaments in epithelial cells.
Cytokeratin 8 typically dimerizes with keratin 18 to form filaments in simple epithelial cells, contributing to structural integrity, signal transduction, and cellular differentiation.
Mutations in this gene are associated with cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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