Thermo Fisher Scientific NHERF1 Polyclonal Antibody
NHERF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 사용 가능. 인간 및 마우스 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 275 and 325 of human Sodium/Hydrogen Exchanger Regulatory Factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
NHERF1 (Na⁺/H⁺ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions.
It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport.
NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth.
It contains a C-terminal ERM-binding region and two N-terminal PDZ domains, enabling intramolecular head-to-tail conformation for interaction regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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