Thermo Fisher Scientific NHERF1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human NHERF1 (EBP50) region 325–358. Suitable for WB, IHC, IHC(P), and IP applications. Predicted to react with mouse, rat, chicken, and bovine. Liquid form, stored at 4°C, shelf life 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 325 and 358 of human Sodium/Hydrogen Exchanger Regulatory Factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Mouse, Rat, Chicken, and Bovine
Target Information
NHERF1 (Na⁺/H⁺ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions. It interacts with various G protein-coupled receptors, regulates their trafficking, and participates in sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is essential for suppressing anchorage-independent growth. It contains a C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ domains, enabling intramolecular head-to-tail conformations that regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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