Thermo Fisher Scientific NHERF1 Polyclonal Antibody
Rabbit 유래의 NHERF1 폴리클로날 항체로 Western blot, IHC, IP 등에 사용 가능. 인간 NHERF1 단백질의 275-325번 잔기 영역을 면역원으로 함. 액상 형태, 4°C 보관, 1년 권장 보관기간. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 275 and 325 of human Sodium/Hydrogen Exchanger Regulatory Factor 1 (NHERF1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
NHERF1 (Na⁺/H⁺ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with β-catenin and is required for its localization at cell-cell junctions.
It interacts with various G protein-coupled receptors and regulates their trafficking, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport.
NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains, allowing it to form head-to-tail intramolecular conformations to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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