Thermo Fisher Scientific NHERF1 Polyclonal Antibody
NHERF1 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, IP 등에 사용 가능. Human 반응성, Mouse·Rat·Chicken·Bovine에도 예측 반응. Antigen affinity chromatography로 정제, 4°C 보관, 연구용 전용.
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Thermo Fisher Scientific NHERF1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 325 and 358 of human Sodium/Hydrogen Exchanger Regulatory Factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Chicken, and Bovine
Target Information
NHERF1 (Na⁺/H⁺ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with β-catenin and is required for its localization at cell-cell junctions. It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM-binding region and two N-terminal PDZ domains, forming head-to-tail intramolecular conformation to regulate interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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