Thermo Fisher Scientific TGM2 Monoclonal Antibody (2A1)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A1

Immunogen

Purified, recombinant, human transglutaminase 2 protein expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1088040

Product Specific Information

A suggested positive control for this product is U87-MG cells.

Target Information

Transglutaminase (TGase) catalyses the crosslink of proteins by forming e-(g-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. In mammals, eight distinct TGase isoenzymes have been identified. Tissue transglutaminase (tTGase), also known as TGase 2, has four distinct domains: N-terminal b-sandwich, catalytic core and two C-terminal b-barrel domains. tTGase may have a role in cell death, cell proliferation, cell differentiation, and receptor-mediated endocytosis. In the Alzheimer`s disease brain, the elevated tTGase activity is manifested by polymerization of a number of proteins, including Ab peptide, b-amyloid precursor protein and the tau protein, with formation of neurofibrillary tangles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.