Thermo Fisher Scientific TGF alpha Polyclonal Antibody, PeproTech
Human TGF-alpha를 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제된 고순도 항체. 세포 성장 및 신호전달 연구에 적합하며 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P16-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TGF alpha Polyclonal Antibody, PeproTech
Thermo Fisher Scientific TGF alpha Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
| Immunostaining (IS) | – | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived Recombinant Human TGF-alpha |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929555 |
Product Specific Information
AA Sequence of recombinant protein:
VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TGF-alpha matrix.
Sandwich ELISA:
To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. In conjunction with PeproTech Biotinylated Anti-Human TGF-alpha (500-P16Bt), it enables detection of at least 0.2–0.4 ng/well of Recombinant Human TGF-alpha.
Western Blot:
To detect Human TGF-alpha by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit for Recombinant Human TGF-alpha is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates proliferation of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independent transformation in cultured cells. Human, murine, and rat TGF-alpha are cross-species reactive. TGF-alpha (aa 50) has 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation, and stimulates cell proliferation. It plays a role in tumor initiation by inducing reversible transformed phenotypes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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