Thermo Fisher Scientific EpCAM Monoclonal Antibody (AUA1)
EpCAM (CD326) 단백질을 인식하는 Mouse IgG1 단일클론 항체로, 다양한 인체 유래 시료 분석에 적합합니다. Western blot, IHC, ICC/IF, Flow Cytometry, ELISA 등 다중 응용 가능. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 |
| Immunocytochemistry (ICC/IF) | 1:10–1:500 |
| Flow Cytometry (Flow) | 1 µg per million cells |
| ELISA | 1:100–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AUA1 |
| Immunogen | LoVo cell line preparation (Human) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2098651 |
Target Information
Ep-CAM (Epithelial Cell Adhesion Molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent adhesion molecule, influencing cell cycle, proliferation, and metabolism via induction of proto-oncogene c-myc and cyclins A and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced epithelial proliferation and is a marker for epithelial-derived tumors. Ep-CAM is expressed on baso-lateral surfaces of most simple epithelia and many carcinomas, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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