Thermo Fisher Scientific Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:50 - 1:100

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

1-5 µg

View 2 publications 2 publications

ChIP assay (ChIP)

1-10 µL

View 4 publications 4 publications

RNA Immunoprecipitation (RIP)

Assay-dependent

-

CUT&RUN (C&R)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Yeast

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4H8

Immunogen

Chemically synthesized phospho-ser 5 YSPTSpPS (Human). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNA pol II CTD,uniProtId:P24928-1,ncbiNodeId:9606,antigenRange:5,antigenLength:1970,antigenImageFileName:MA1-46093_RNA_pol_II_CTD_P24928-1_House_mouse.svg,antigenImageFileNamePDP:MA1-46093_RNA_pol_II_CTD_P24928-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1018366

Product Specific Information

MA1-46093 reacts with DNA-directed RNA Polymerase II Largest Subunit in human, yeast and mouse samples. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology.

MA1-46093 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, IHC (P) and Immunoprecipitation applications.

The MA1-46093 immunogen is 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). This antibody detects both the phosphorylated and non-phosphorylated forms.

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.